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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in lots of tumors, which results in chromosome instability, and thus could add to tumorigenesis. This protein was observed to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant purpose in marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already described. Two extra splice variants have been noted but thorough nucleotide sequence facts just isn't however readily available.
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Blocking peptides are peptides that bind specifically towards the focus on antibody and block antibody binding. These peptide commonly has the epitope regarded via the antibody.
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This guide outlines even further achievable optimization of mobile and immunoassay parameters to be sure the best possible benefits are...
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This guide outlines additional feasible optimization of mobile and immunoassay parameters to ensure the absolute best final results are...
Antibodies certain to the blocking more info peptide no longer bind to your epitope around the concentrate on protein. By evaluating the staining in the blocked antibody versus the antibody by yourself, you can see which staining is specific.
Goal Details Cyclin E belongs towards the extremely conserved cyclin loved ones, whose associates are characterised by a spectacular periodicity in protein abundance through the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation patterns which contribute into the temporal coordination of every mitotic event. Cyclin E sorts a fancy with and features for a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary which is degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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